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WBC differential channel

Analysing differentials consists of a cytochemical reaction of the cells with a reagent, followed by a fluorescence flow cytometric analysis.

A surfactant in the reagent induces the lysis of red blood cells. At the same time, cell membranes of white blood cells are perforated with ultramicroscopic pores through which a polymethine-based fluorescence marker migrates and binds to nucleic acids. This prepared sample is then analysed using fluorescence flow cytometry.

The measurement signals related to side scatter (SSC) and side fluorescence (SFL) are analysed and depicted in a scattergram.  Cells with similar cytochemical properties fall within the same area in the scattergram and can be separated using an advanced software algorithm.

The differential channel provides counts of all normal white blood cell subpopulations as well as flag information in cases of abnormalities.

Fluorescence flow cytometry

RET channel

DC sheath flow detection method

SLS detection
method

Analyser channels

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