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CyFlow® Ploidy Analyser

  • Screening for Haplo-, Diplo-, Triplo- and Polyploids
  • Ploidy and Genome size analysis in less than two minutes
  • Detection of Anisoploids, Allopolyploids and Aneuploids
  • No time consuming microscopic evaluation
  • Any plant material can be analysed: leaves, seedlings, roots, flowers, peel of fruits, seeds

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Automatic Measurement of Ploidy Level and Genome Size in Plants and Animals

High-performance flow cytometry

The CyFlow Ploidy Analyser is a compact flow cytometer for ploidy analysis, high-resolution DNA and genome size analysis for plants, animals and micro-organisms.

This modular system is equipped with a UV LED (365 nm) and/or a green laser (532 nm) which, when used in combination with Partec CyStain reagents, provides high-resolution DNA analysis with DAPI or propidium iodide.

The CyFlow Ploidy instrument measures the DNA content of many thousands of individual nuclei within seconds or minutes. The result is directly displayed as a DNA histogram on the internal 15.4" colour display. This method is time- and cost-saving as it provides precise results quickly with an efficient and validated workflow. For higher throughput, it is possible to equip the system with an Autoloading Station that accepts up to two 96-well plates or 120 test tubes as a single load.

Flow Cytometry

Specification

Specification

Light sources:

Up to two light sources

  • UV LED (365 nm)
  • Nd-YAG green laser at 532 nm (30 mW)

 

Optics:

  • Standard set-up and filters for propidium iodide (long pass filter 590 nm) and/or DAPI/SSC (long pass filter 435 nm)

 

Flow system:

  • Quartz flow cuvette for laminar sample transport and hydrodynamic focussing
  • True Volumetric Absolute Counting (TVAC) based on mechanical volume measurement
  • Completely closed fluidic system
  • Sample port with biosafety cleaning system

 

Software:

  • Windows™-based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
  • Peak analysis function

 

Dimensions:

  • Stand-alone instrument: W 385mm x H 290mm x D 280mm
  • With Autoloading Station: W 745mm
  • With screen open: H 528mm

 

 

Software

Software

CyView: flow cytometry data acquisition and analysis software

  • Windows™-based FCM software CyView for real-time data acquisition, real-time data analysis and real-time data display
  • Easy set-up of experimental templates (configuration files)
  • Flow Cytometry Standard (FCS) data format for storage of original and evaluated data
Upgrades

Upgrades

CyFlow Robby 6 Autoloading Station

General facts:

  • Protective housing with no light incidence 
  • Flexible well/tube measurements

 

Microplates and tubes:

  • 48- or 96-well plates, V-, U- and flat-bottom are fitting (max. two) 
  • Plates meeting the standards ANSI/SLAS (1-2004 through 4-2004) 
  • 2mL-tubes (max. 120)

 

Autoloader dimensions:

  • W 360mm x H 270mm x D 370mm
Applications

Applications

Plant and animal ploidy determination

Aneuploidy detection

Precise genome size estimation

 

For research and industrial applications:

  • Biology and Biotechnology
  • Seed and seedling industry
  • Plant taxonomy and population evolution / ecology
Complementary products

Complementary products

Sample preparation

  • CellTrics
  • CyStain UV Ploidy (DAPI)
  • CyStain UV Precise P (DAPI)
  • CyStain UV Precise P automate (DAPI)
  • CyStain UV Precise T (DAPI)
  • CyStain UV Precise T automate (DAPI)
  • CyStain DNA 1 step (DAPI)
  • CyStain DNA 2 steps (DAPI)
  • CyStain PI Absolute P (PI)
  • CyStain PI Absolute T (PI)

 

 

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